Why must the concentration of L-tryptophan be kept to a relatively low level when culturing mammalian cells?

It's an essential amino acid, so I cannot fathom why low concentrations as opposed to higher ones are best. Does it have to do with carcinogenesis or some other dangerous side effect I wonder?

  • $\begingroup$ It is essential but it is also not required in huge amounts. I am not sure if it has toxic effects. $\endgroup$
    Aug 14, 2015 at 13:22
  • $\begingroup$ I just assumed it was due to feedback regulation that would affect other amino acids' production, but I really don't know. It's a good question. $\endgroup$
    – johntreml
    Aug 17, 2015 at 16:41


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