0
$\begingroup$

![enter image description here][1]

What was possibly wrong in my gel electrophoresis when I didn't see bands of DNA ladder? I'm running a 2% electrophoresis gel (65v for 2h) to confirm the presence of qPCR amplification products, I can see the gDNA bands, is only the ladder that don't appear.

$\endgroup$

closed as too broad by AliceD, March Ho, WYSIWYG Aug 16 '15 at 17:25

Please edit the question to limit it to a specific problem with enough detail to identify an adequate answer. Avoid asking multiple distinct questions at once. See the How to Ask page for help clarifying this question. If this question can be reworded to fit the rules in the help center, please edit the question.

  • 4
    $\begingroup$ Try a new batch of ladder, rerun the gel. Without more info this question cannot be answered. $\endgroup$ – AliceD Aug 14 '15 at 23:02
  • 2
    $\begingroup$ Can you add a pic? $\endgroup$ – rhill45 Aug 15 '15 at 0:26
  • $\begingroup$ Already added, the ladder should appear in the first lane. $\endgroup$ – Carina Aug 17 '15 at 14:16
  • 1
    $\begingroup$ did you try rerunning with new ladder? $\endgroup$ – AliceD Aug 17 '15 at 14:18
  • $\begingroup$ no, this was the last ladder available in the lab with the base pairs i needed. $\endgroup$ – Carina Aug 17 '15 at 19:28
6
$\begingroup$

As one attempts their daily Sisyphean challenge of uncovering a new fact there is one truism, or platitude, that informs all of our efforts at troubleshooting an unanticipated experimental outcome. This can be paraphrased as:

The number one cause is pilot error.

Accumulated experience of bench scientists everywhere shows that their own human errors are the simplest explanation whenever they try to deduce what could have gone wrong. In the real world this translates into an operating axiom:

Don't waste time and energy coming up with new physical laws, or multi-step explanations that require you to remake and test all of your reagents, just repeat the experiment and see if this phenomenon is reproducible.

With that new information, that it is, or is not reproducible, you can decide if there is any hypothesis to test. But please, don't just take my word for it. Survey experimentalists, and the more experience they have the better.

$\endgroup$

Not the answer you're looking for? Browse other questions tagged or ask your own question.