I'm dealing with two different protein(say, protein_a and protein_b) which stays in an interacting-oligomeric form in biological system.
I have so far successfully been able to purify both the proteins using IMAC(Ni-NTA) protocol.
Further TEM analysis of the purified protein is showing the presence of oligomeric form(for each of them separately) in the purified sample.
But, how can I be able to detect or show how the oligomers of protein_a and protein_b is interacting to produce a much larger macromolecular entity ? And how can I be able to quantify that interaction ?
Any ideas regarding this issue would be highly appreciated.