When human-rodent hybrids are made the amount of human chromosome in the hybrid can be reduced by irradiation. Why and how exactly is this irradiation step performed?

  • $\begingroup$ "Why and how exactly is it performed?" Are you specifically asking about irradiation? $\endgroup$ – James Aug 25 '15 at 17:37
  • $\begingroup$ Im asking chiefly why it is done $\endgroup$ – Naveen Kumaar Aug 25 '15 at 17:40
  • $\begingroup$ And the principle behind it $\endgroup$ – Naveen Kumaar Aug 25 '15 at 17:41
  • $\begingroup$ I've made an edit that tries to specify that a bit better. $\endgroup$ – James Aug 25 '15 at 17:49

Human cells are irradiated with X rays (to fragment the DNA) and then fused with rodent cells. This is useful for mapping because closely linked markers are more likely to appear in the same hybrid: there is a lower probability of radiation induced breakage between the markers because they are close together.


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