I am studying a non-cultivable bacterium living in an insect host, and I would like to generate some random mutants of this bacteria while preserving the insect.

I am wondering if one way to do so might be to use directed mutagenesis directed towards the bacterial polymerase, which would leads it to increase the error rate.

  • Does any one has already seen a study with this kind of approach?
  • Does any one knows or guess another way to obtain some random mutants of the bacteria while preserving the host insect?

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