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I'm looking for suggestions as my internet searches aren't turning up much.

I'm trying to find a plasmid that has the pBR322 origin, as it's low(ish) copy number and can tolerate large inserts. Additionally I need the plasmid to be inducible with a system other than the pBAD setup (e.g. IPTG would be fine, but if there is one that is more tightly repressed that would be preferrable as IPTG is leaky).

Lastly, I could do with it having a potentially obscure resistance cassette, but this is less of a problem.

Any help appreciated.

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The pET vectors family is what you are looking for.

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  • $\begingroup$ Just to add, if you're worried about leaky expression you can select a strain expressing T7 lysozyme from pLysS. $\endgroup$ – canadianer Jul 17 '17 at 14:47

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