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I am interested in positive charged polyacrylamide to electrophorese molecules I am interested in.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2643323/ http://onlinelibrary.wiley.com/doi/10.1002/bip.360300309/pdf

The articles shown above use allyltriethylammonium bromide (ATAB), but I am not sure which chemical they mention.

Or is there any other chemical to add to acrylamide to get positive charged polyacrylamide copolymer?

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    $\begingroup$ I would say that this question is better suited for chemistry.se. $\endgroup$ – Chris Sep 27 '15 at 9:45
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ATAB contains a quaternary amine and a terminal carbon-carbon double bond. The polyacrylamide reaction links acrylamide by their double bonds forming the polymer. The double bond on ATAB can be incorporated into the growing polymer chain, and the quaternary amine holds a positive charge, making a positively charged polymer.

The immobilines mentioned are probably just a weak acid or weak base group with a terminal double bond like ATAB or acrlyamide. They get trapped into the polymer and if you pour the gel with a pH gradient, they pick up charges according to the pH. I haven't found a good concrete immobiline example, I can't access that second article you linked.

I am away from my good computer and cannot make chemical structures right now.

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  • $\begingroup$ Thank you. I sensed how it works when I read, but I was not able to find the commercial products so I was wondering why. Now I find it. $\endgroup$ – 243 Sep 27 '15 at 15:03

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