Well, i would like to enrich bacterial and archaeal ammonia oxidisers from soil samples using Minimal Salt media. They are slow growing microbes. I would like to enrich them with the aim to produce pure cultures.
I have always used conical flasks for enrichment as they provide better aeration, surface area, better distribution of nutrients, etc. However, due to some unavoidable reason, i would like to find an alternative to conical flask. Is it fine to use large test tubes?