I have very limited knowledge when it comes to DNA fingerprinting. I know about the technique gel electrophoresis and how everything is carried out. But I don't know how to read the result or understand that. Can anyone please explain me how to read the result [profile of bands]?
Because of my lack of knowledge, I can't solve problems like this one. Please explain me using this one so that I can get a clear idea about DNA profiling. This seems like home-work problem but the thing is I want to understand the technique of restriction mapping by reading the result of restriction digestion on electrophoresis and my intention is to understand the topic. Please Help.
And I can't describe how I tried to solve this question because I don't have any kind of knowledge about this technique [Restriction Mapping] so my efforts are very silly to mention and I have improper way of approach to this as I don't know how to do the Restriction Mapping.
But I am given the answer which I tried to make sense by working on it but I just couldn't help it. It seems easy after seeing the Map but I don't get the relation between the profile of bands and the Map so if I am given a different problem I will fail to come up with a reasonable answer.
What I am trying to say is, by seeing this map and working on it I get to agree that this is the correct mapping for the data given but I have some doubts, like, why we keep 3Kb distance between BamHI and EcoRI sites. Working out in some another way will end up with wrong answer. How these inticacies are understood? By reading the profile of bands [data given] right? So that's what I am asking for you to explain to me. I don't know anything about it.
Thank you always.