I quantify the enzyme (polygalacturanase) activity by DNSA method. To determine endo-polygalacturonase activity I'm searching for a good easy enzyme extracting method. Any suggestions for a suitable buffer?
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$\begingroup$ Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025. The authors use a sephacryl S-100 gel filtration column (chromatography), followed by enzyme activity assay and SDS-PAGE. $\endgroup$ – CKM Oct 10 '15 at 18:24
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