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I have to study my peptide's folding on membrane mimetic (model membrane) by circular dichroism spectra. Now I'm looking for suitable methods for preparation of vesicles: LUV, SUV, GUV- large, small and giant unilamellar vesicles.

I have studied several methods but I am having a hard time grasping the key factors that affect the resulting vesicle size (LUV, SUV, GUV, etc.)

Any pointers are greatly appreciated.

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Giant unilamellar vesicles (GUVs) are made by methods like hydration of phospholipid membrane generally by use of some solvent , use of electric current, combining small vesicles etc. large unilamellar vesicles (LUVs) are made by methods like evaporation with low concentration of phospholipid, dialysis etc. Small unilamellar vesicles (SUVs) are made by methods Injecting solution spontaneously, microfludic methods etc. There are many methods reviewed extensively (just search in pubmed you will get lot of them)

Now coming to your question, factor which affect sizes are depends on method you are using. I will list out few, rest you can check in references of each method I gave above. Most of times these methods will have all kinds of vesicles. Success of these methods relies on which method gives you highest proportion of vesicle size you desired.

Besides all of this, basic physics behind this explained here like energy difference shown below (Image taken from (Lasic 1988))

enter image description here

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