I am going to prepare a c-myc fusion protein with the following configuration:
(28-residue signal sequence)-(c-myc)-(GGSGGGSG Linker)-(Protein of Interest (POI))
POI is a transmembrane protein and the 28-residue signal sequence is needed for membrane incorporation in ER.
My concern is the disruption of the c-myc/anti-myc Ab interaction due to the fact that c-myc being fused on both ends. Does anyone have any experience or reference with a similar construct?