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I read that this proteins lack of slicer activity, so what mechanism do they use to perform gene regulation?

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  • $\begingroup$ Just the presence of a large protein complex in the 5'-UTR of an mRNA can prevent ribosome binding and therefore translation. I'm not saying that that is the actual mechanism, but it's plausible. $\endgroup$ – canadianer Oct 12 '15 at 20:38
  • $\begingroup$ Ago(s) of what organism? I presume you are talking about mammalian Ago. I am asking this because Ago proteins with same name may not be orthologs; for e.g. Drosophila Ago3 (along with Piwi and Aub) belongs to the Piwi family which in vertebrates includes Piwi, Piwi-like and Piwi-2. Also, there are differences between Ago1&2 of Drosophila and mammals. Plants have many Ago proteins (even C.elegans) $\endgroup$ – WYSIWYG Oct 13 '15 at 5:26
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Paper by Zinovyev and colleagues in the paper "Mathematical modeling of microRNA‐mediated mechanisms of translation repression" find that there are reports to 9 different mechanisms of miRNA-regulation.

Here we are reviewing available experimental data on all reported mechanisms of microRNA action, grouping them in a way which elucidates the main details supporting each of these proposed mechanisms.

Here is a list of the mentioned mechanisms:

M1: Cap-40S Initiation Inhibition

M2: 60S Ribosomal Unit Joining Inhibition

M3: Elongation Inhibition

M4: Ribosome Drop-Off

M5: Co-translational Nascent Protein Degradation

M6: Sequestration in P-Bodies

M7: mRNA Decay

M8: mRNA Cleavage

M9: Transcriptional Inhibition Through microRNA-Mediated Chromatin Reorganization Following by Gene Silencing

Also here is a link to the Pubmed entry of the article:

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  • $\begingroup$ Perhaps you could briefly summarize the mechanisms. $\endgroup$ – canadianer Oct 12 '15 at 20:58
  • $\begingroup$ And could you add a proper citation with a web link? -1 $\endgroup$ – AliceD Oct 12 '15 at 21:28

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