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I am trying to silence the tph-1 (tryptophan hydroxylase) gene in C. elegans using the pLT63 plasmid to check if that particular gene has anything to do with the pharyneal pumping or not. Am I using the right plasmid for the purpose?

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"pLT63 contains 0.8 kb of the fem-1 gene inserted into the L4440 vector" (1). The gene fem-1 is involved in determining the male sexual trait in C. elegans (you can verify this on uniprot). It may be best, however, to stick to a "normal" plasmid like L4440 that'll just have your tph-1 insert ligated in.

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  • $\begingroup$ Is there any direct link between tph-1 and pharyngeal pumping in the worms that you know of? $\endgroup$ – MPG Oct 27 '15 at 0:40
  • $\begingroup$ I did read through some literature and while some evidence says tph-1(mg280), a deletion of an allele, alters the ability of the worms to fine-tune the speed of their feeding, i think that was here. But overall, and we see in this study, the worms have relatively normal feeding. I haven't done the experimentation myself, however, so I can't 100% make a claim here. $\endgroup$ – CKM Oct 27 '15 at 0:54
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    $\begingroup$ Check out the WormBase site at www.wormbase.org. Just type tph-1 in the search box on the right hand side at the top of the page. $\endgroup$ – mdperry Oct 27 '15 at 11:00

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