I am trying to silence the tph-1 (tryptophan hydroxylase) gene in C. elegans using the pLT63 plasmid to check if that particular gene has anything to do with the pharyneal pumping or not. Am I using the right plasmid for the purpose?


"pLT63 contains 0.8 kb of the fem-1 gene inserted into the L4440 vector" (1). The gene fem-1 is involved in determining the male sexual trait in C. elegans (you can verify this on uniprot). It may be best, however, to stick to a "normal" plasmid like L4440 that'll just have your tph-1 insert ligated in.

| improve this answer | |
  • $\begingroup$ Is there any direct link between tph-1 and pharyngeal pumping in the worms that you know of? $\endgroup$ – MPG Oct 27 '15 at 0:40
  • $\begingroup$ I did read through some literature and while some evidence says tph-1(mg280), a deletion of an allele, alters the ability of the worms to fine-tune the speed of their feeding, i think that was here. But overall, and we see in this study, the worms have relatively normal feeding. I haven't done the experimentation myself, however, so I can't 100% make a claim here. $\endgroup$ – CKM Oct 27 '15 at 0:54
  • 1
    $\begingroup$ Check out the WormBase site at www.wormbase.org. Just type tph-1 in the search box on the right hand side at the top of the page. $\endgroup$ – mdperry Oct 27 '15 at 11:00

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.