I have got two different sized colonies in a plate of Salmonella Paratyphi, identified by biochemical methods. What does two different sized colonies mean? What kind of question can I investigate in this regard?
Positivity of serum sample was checked in BacT/Alert blood culture system. Bacteria was subcultured from the positive result giving culture bottle from the BacT/Alert system and incubated overnight at 35 degree celsius. Two different sized colonies were found and they were subjected to biochemical and antisera tests and both identified as Salmonella Paratyphi.