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I want to identify potential conserved Acetylation sites in protein X. As it is known, acetylation happens in Lysines (K), thereby I would like to identify conserved K residues for protein X during evolution. Eventually I would use different Acetylation site prediction tools to further filter the results, but initially I need the other data, does anyone know a user friendly but trust worthy bioinformatic tool to do so?

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You can use the NCBI subpage HomoloGene

Halfway down page on left you can choose to show Multiple Alignment as seen in this screenshot.

Pic

I think you will then see what you are looking for as in this screenshot:

enter image description here

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