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UACC-462 is a pancreatic cell line established in the University of Arizona Cancer Center (UACC). The cells are available from ATCC and there is the instruction for culturing UACC-462.

M-41 medium. The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium (final conc.):

fetal bovine serum to a final concentration of 5%

0.01 mg/ml transferrin

0.01 mg/ml insulin

5 µg/ml (55 U/ml) catalase

3.6 µg/ml (0.01 mM) hydrocortisone

10 ng/ml EGF

3 ng/ml Estradiol

70 µg/ml O-phosphorylethanilamine

0.0008 µg/ml L-thyroxine

extra 2mM L-glutamine

Atmosphere: air, 95%; carbon dioxide (CO2), 5%. Temperature: 37°C

On the other hand, L-15 is designed to culture without CO2 and often used time-laps experiments under microscope.

My question is why L-15 based media can be used in CO2 incubator. Is the cell line acid-philic?

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  • $\begingroup$ There are a number of cancer cell lines that are grown in various forms of L-15 in 5% $CO_2$ incubators. I don't know the exact biochemistry behind it, but it works, so that's what's used. $\endgroup$ – MattDMo Nov 13 '15 at 17:03
  • $\begingroup$ @MattDMo Regarding this cell line, an ATCC technician admitted the mistake. Do you have experiences culturing cancer cells with L-15 based media in 5% CO2? I would like to know which cells can be grown under the condition, for future reference. $\endgroup$ – 243 Nov 13 '15 at 17:24
  • $\begingroup$ unfortunately, I am no longer at the lab where they did this, so I can't check the database. So, ATCC said the line shouldn't be cultured with $CO_2$? $\endgroup$ – MattDMo Nov 13 '15 at 17:27
  • $\begingroup$ @MattDMo "It should read 100% atmospheric conditions." That's what they said. $\endgroup$ – 243 Nov 13 '15 at 17:32
  • $\begingroup$ @MattDMo May I post another question to ask cells that can be grown with L-15 based media in 5%CO2? $\endgroup$ – 243 Nov 13 '15 at 17:36

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