M-41 medium. The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium (final conc.):
fetal bovine serum to a final concentration of 5%
0.01 mg/ml transferrin
0.01 mg/ml insulin
5 µg/ml (55 U/ml) catalase
3.6 µg/ml (0.01 mM) hydrocortisone
10 ng/ml EGF
3 ng/ml Estradiol
70 µg/ml O-phosphorylethanilamine
0.0008 µg/ml L-thyroxine
extra 2mM L-glutamine
Atmosphere: air, 95%; carbon dioxide (CO2), 5%. Temperature: 37°C
On the other hand, L-15 is designed to culture without CO2 and often used time-laps experiments under microscope.
My question is why L-15 based media can be used in CO2 incubator. Is the cell line acid-philic?