I used BLAST to find the best aligned DNA sequence to my query sequence. The BLAST result shows a number of sequences that it calculated to be the best aligned sequences to the query sequence that I give. Each of those aligned sequences have an e-value score representing the best hit by chance.
My question is how can I determine if the query sequence is highly conserved with the aligned sequence, or not? Can I determine that by saying the sequence with best hit e-value (lower e-value) is the one that is highly conserved? or are there other concepts that I need to consider in this case?
I appreciate your help!