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I've been looking for the sequence of pPROLar.A122 plasmid but I haven't found it. Where could I find the sequence?

The first link (addgene) on googling reports a plasmid which is reported to have the pPROLar.A backbone. However, this is a modified version with Ampicillin and LuxR. I'm trying to reconstruct it from it and another plasmid.

The plasmid map looks like this:

enter image description here

Thanks!

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  • $\begingroup$ This is not. pPROLar.A122 plasmid looks like this: apps2.bvl.bund.de/vectorwww/image.do?id=427 and this is a modified version with Ampicillin and LuxR. But I'm trying to reconstruct it from it and another plasmid. $\endgroup$ – Carlos Toscano-Ochoa Nov 24 '15 at 15:01
  • $\begingroup$ The problem is the reliability of reconstructed sequences. If a problem arrives with the cloning maybe was because of that. I've already searched the whole Google, that's the reason because I asked here. $\endgroup$ – Carlos Toscano-Ochoa Nov 24 '15 at 15:04
  • $\begingroup$ I am afraid the sequence is not available anywhere. You should write to Clontech to ask for it. If you do get a reply then please post it here. It will be useful for others. $\endgroup$ – WYSIWYG Nov 25 '15 at 5:40
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The pPROLar.A112 sequence is:

CCATGGGTGA ACAGAAACTG ATCAGCGAAG AGGATCTGGG TGGAGATGAC GATGACAAGG TGGTCGACAA GCTTGGATCC CTGCAGGCCT CAGGGCCCGA TCGATGCGGC CGCTTAATTA ATTAATCTAG AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGC CCTAGACCTA GGGGATATAT TCCGCTTCCT CGCTCACTGA CTCGCTACGC TCGGTCGTTC GACTGCGGCG AGCGGAAATG GCTTACGAAC GGGGCGGAGA TTTCCTGGAA GATGCCAGGA AGATACTTAA CAGGGAAGTG AGAGGGCCGC GGCAAAGCCG TTTTTCCATA GGCTCCGCCC CCCTGACAAG CATCACGAAA TCTGACGCTC AAATCAGTGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGCGG CTCCCTCGTG CGCTCTCCTG TTCCTGCCTT TCGGTTTACC GGTGTCATTC CGCTGTTATG GCCGCGTTTG TCTCATTCCA CGCCTGACAC TCAGTTCCGG GTAGGCAGTT CGCTCCAAGC TGGACTGTAT GCACGAACCC CCCGTTCAGT CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGAA AGACATGCAA AAGCACCACT GGCAGCAGCC ACTGGTAATT GATTTAGAGG AGTTAGTCTT GAAGTCATGC GCCGGTTAAG GCTAAACTGA AAGGACAAGT TTTGGTGACT GCGCTCCTCC AAGCCAGTTA CCTCGGTTCA AAGAGTTGGT AGCTCAGAGA ACCTTCGAAA AACCGCCCTG CAAGGCGGTT TTTTCGTTTT CAGAGCAAGA GATTACGCGC AGACCAAAAC GATCTCAAGA AGATCATCTT ATTAATCAGA TAAAATATTA CTAGATTTCA GTGCAATTTA TCTCTTCAAA TGTAGCACCT GAAGTCAGCC CCATACGATA TAAGTTGTTA CTAGTGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA GGAGTCCAAG CGAGCTCTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGGTCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCGCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCCT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CAGATCTTGA TCCCCTGCGC CATCAGATCC TTGGCGGCAA GAAAGCCATC CAGTTTACTT TGCAGGGCTT CCCAACCTTA CCAGAGGGCG CCCCAGCTGG CAATTCCGAC GTCTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG GCCCTCGGAC ACCGAGGAGA ATGTCAAGAG GCGAACACAC AACGTCTTGG AGCGCCAGAG GAGGAACGAG CTAAAACGGA GCTTTTTTGC CCTGCGTGAC CAGATCCCGG AGTTGGAAAA CAATGAAAAG GCCCCCAAGG TAGTTATCCT TAAAAAAGCC ACAGCATACA TCCTGTCCGT CCAAGCAGAG GAGCAAAAGC TCATTTCTGA AGAGGACTTG TTGCGGAAAC GACGAGAACA GTTGAAACAC AAACTTGAAC AGCTACGGAA CTCTTGTGCG TAAGGAAAAG TAAGGAAAAC GATTCCTTCT AACAGAAATG TCCTGAGCAA TCACCTATGA ACTGTCGACT CGAGCATAGC ATTTTTATCC ATAAGATTAG CGGATCTAAC CTTTACAATT GTGAGCGCTC ACAATTATGA TAGATTCAAT TGTGAGCGGA TAACAATTTC ACACAGAATT CATTAAAGAG GAGAAAGGTA C

It was sent to me directly from Takara Bio.

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