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I recognize some interesting patterns when analysing DNA methylation of BEAS-2B cells. Does anybody know, if the cell culture is female or male? I already asked the customer support of Sigma Aldrich without success.

Information on the official website just mention "non-cancerous individuals" as source organisms.

http://www.sigmaaldrich.com/catalog/product/sigma/95102433?lang=de&region=DE

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The cell line is most likely genetically male. On the English (US) site, the Description box has several sections, of which one is DNA Profile. In there, it lists amelogenin as X, Y. This gene is located on both the X and Y chromosomes (AMELX and AMELY, respectively), which have distinct PCR-detectable sequence differences. It is often used in helping to determine the gender of unknown samples in the realm of forensics - it is not 100% accurate, so other genetic tests (such as for SRY) are also performed.

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  • $\begingroup$ My DNA methylation data don't show a clear male oder female status. There is definitly an observable "male" effect, but a clear classification is not possible with my dataset. The results indicate a mixture. Anyway, I will remove gonosomal probes from further analyses. Thanks for your answer! $\endgroup$ – easelpeasel Dec 3 '15 at 2:14

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