If I have some synthetic DNA sequence (<=20 bp long), is there a way for me to reliably insert this sequence next to some n-bp motif? I'd like for this to be possible in humans. If so, are there any restrictions on the size of n?
I have explored the use of CRISPR/Cas9 for this end, but it is limited in the sense that the gRNA target sequence must be adjacent to a PAM sequence. Attempts have been made to solve this problem (http://www.nature.com/nature/journal/v523/n7561/full/nature14592.html#affil-auth), but they fall short of full generality for n. Are there any better approaches?