I have been asked a general question: Once I have cloned a full-length cDNA into an expression vector, what final important control must I do before I transfect this into an embryonic stem cell line?

The protein involved already has an N-terminal FLAG tag...could this mean that a positive control is needed where the cell is cotransfected with a vector carrying say GFP and then measuring rate of transfection? Or could the cDNA be converted to mRNA which then acts as a positive control? I am not very familiar with transfection procedures so could someone please point me explain this problem to me, thanks!


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.