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I have been asked a general question: Once I have cloned a full-length cDNA into an expression vector, what final important control must I do before I transfect this into an embryonic stem cell line?

The protein involved already has an N-terminal FLAG tag...could this mean that a positive control is needed where the cell is cotransfected with a vector carrying say GFP and then measuring rate of transfection? Or could the cDNA be converted to mRNA which then acts as a positive control? I am not very familiar with transfection procedures so could someone please point me explain this problem to me, thanks!

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