Those plants which are rich in secondary metabolites and polysaccharides DNA isolation can be done using certain chaotropic agents? can any one explain how it works?

  • $\begingroup$ Chaotropes would disrupt protein structure. Maybe they help by removing the DNA binding proteins? $\endgroup$ – user137 Jan 12 '16 at 9:24

Chaotropic agents are cosolutes that can disrupt the hydrogen bonding network between water molecules and reduce the stability of the native state of proteins by weakening the hydrophobic effect. a chaotropic agent reduces the amount of order in the structure of a protein formed by water molecules, both in the bulk and the hydration shells around hydrophobic amino acids, and may cause its denaturation (biochemistry). A chaotropic agent is often used in DNA isolation. It's purpose is

a. To degrade membrane lipids

b. To destroy the endoplasmic reticulum

c. To degrade mitochondria

d. To limit the amount of magnesium salts in the lysate

e. To destroy the three dimensional structure of proteins

To make it simple, a chaotropic agent destroys the 3D structure of a protein, thereby leading to subsequent protein degradation. It serves as a proteinase enzyme, cleaning the cellular proteins in your DNA isolation and purification process.



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  • $\begingroup$ Thanks a lot for valuable answer..... but does it also any effect on removing secondary product or lipid or polysachharides $\endgroup$ – P Josh Jan 25 '16 at 8:01
  • $\begingroup$ Nucleic acids share many structural similarities with polysaccharides, and they will therefore co-purify through most fractionation steps. $\endgroup$ – mdperry Mar 17 '16 at 0:44

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