I'm looking for both simple and complex assays or technologies than can be used to determine if two competing molecules are competitive or noncompetitive. I figure xray crystallography is a clear one, but say I can't get crystals to form. What other techs or assays could I use to determine this?
It's a protein-protein interaction with a small molecule competing with one of the proteins. This particular set has not been done before. All interactants are purified.