I've been working as the computer guy for a microbiology lab for the past few months. I've always been interested in bench work, but my wet lab experience is rather limited and thus so is my understanding of wet lab techniques.
The PI has expressed his support and given me the opportunity to conduct my own project from start to finish (generation of wet lab data and computational analysis) and write a paper in preparation for my Ph.D school applications.
I realize that coming up with an actual question is a long and thought out process so I want to do my homework before approaching him with a question. I've read quite a bit of literature and have come up with a question i'd like to answer, the computational stuff I can handle and explain in detail, but I'm having a hard time with the wet lab techniques I could use to generate some of the data i'm interested in.
Effectively I am looking to obtain a list of potential cellular transcription factors that are physically associated (bound) with/by a specific viral protein. Is there a lab technique that would allow me to generate such a list?
Links to papers, wikis, your own theories or anything will be greatly appreciated. Also any advice, or "this isn't possible" is all appreciated, as long as you can give me a bit of "this is why".
Thank you! (PS I'm not sure if this type of post is allowed here).
EDIT: It is important to note that I am looking for new binding partners.