One of the key rules in primer designing is that Tm (melting temperature) of forward and reverse primers should be in the range of ±5°C. For example, if Tm of forward primer is 62°C, then reverse primer should be either 67°C or 57°C.

What happens if both the primers are having same Tm?

Is this to be considered as perfect score of finding correct primers set?



1 Answer 1


It is actually good if both the primers have the same Tm (Melting Temperature) because you would basically want both primers to anneal at your Ta (Annealing Temperature) which is generally set as Tm-5. If the Tm(s) don't match, then your Ta should be set according to the lower Tm.


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