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I have seen the term "mouse LS cell" used in several papers now, but I haven't found an explanation for the origin of these cells (for example, this paper).

What are mouse LS cells? What is their origin (besides coming from mouse!)?

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Not an easy question, but I got some references about it. Mouse LS cells are Fibroblasts growing in suspension culture, the paper in reference 1 has this paragraph in its Materials and Methods section:

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This cell line seems to be in culture since the 1940s, as the reference to Sanford, Earle and Likely, 1948 suggests. Digging up this reference (number 2 below) tells a bit more about the isolation:

The cells of this study were from Earle's strain L (6), which originated from normal, subcutaneous, connective tissue of an adult C311 strain mouse.

Reference 6 in the Sanford et al. paper is:

EARLE, W. R.: Production of malignancy in vitro. IV. The mouse fibroblast cultures and changes seen in the living cells. J. Nat. Cancer Inst, 4: 165-212 (1943).

which gives no further details. It is a methodic paper which analyzes the conditions necessary for cell culture - interesting on a historical point of view, since the techniques where developed at that time. The full PDF is linked in reference 3.

About the name I need to speculate a bit, but since the original cells where named "Strain L", and LS cells where derived from there to grow in suspension, it might translate into Strain L Suspension cells.

References:

  1. The Uptake of Amino Acids by Mouse Cells (Strain LS) During Growth In Batch Culture and Chemostat Culture: The Influence Of Cell Growth Rate
  2. The Growth in Vitro of Single Isolated Tissue Cells
  3. Production of Malignancy in Vitro.
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