Suppose one has extracted RNA from a plant, converted it to the corresponding cDNA & amplified it but now wants to cut out a particular, already-sequenced gene out from it, how does one proceed?
i.e. How does one cut out that particular segment of the whole DNA which one wants? Does one always find restriction sites (or rather cleavage sites) at both ends of the desired gene segment & then one treats the cDNA sample with those specific restriction enzymes?
To get the gene shown below I'd find an enzyme that cuts at the GGCCAC site and another to cut at AAAAAA?