0
$\begingroup$

Major intron boundary nucleotides are GU-AG. Minor intron boundary nucleotides are AT-AC. Why does the former use RNA Uracil and the latter use DNA thymine? Since introns are in the primary RNA transcript, shouldn't both be RNA and use U rather than T?

$\endgroup$
2
$\begingroup$

It is not unusual for RNA sequences to be reported with T instead of U; the writer is reporting the cDNA sequence instead of the RNA sequence. In RNA at the boundary of the minor intron, there is a uracil. In the following page, the writers follow the oft-used convention of describing RNA with T instead of U (writing the cDNA sequence). https://en.wikipedia.org/wiki/Minor_spliceosome

$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.