0
$\begingroup$

I had ethanol precipitated a large amount of DNA (2ml) and had to split the sample in half to spin down because only the microcentrifuge has the correct rotor to spin that fast. I want to get as concentrated of a DNA sample as possible. Is it possible (correct) to combine the resuspended DNA? I wanted a total of 30 ul of extracted DNA. Could I resuspend each of the two sample in 15 ul and then combine them?

What is the best way to go about this. I realize just keeping them separate might be the best option. I also realized I could've lost DNA when transferring the sample to a smaller tube. I did this because I don't believe it is correct to spin for longer at a lower speed...

$\endgroup$
3
  • $\begingroup$ Assuming you are using standard 1.5ml microcentrifuge tubes, it is completely possible to resuspend a dried DNA pellet from an ethanol/isopropanol precipitation. I have resuspended RNA pellets in 10µl water frequently with no issues. $\endgroup$
    – March Ho
    Commented Mar 8, 2016 at 15:05
  • $\begingroup$ But then can I combine the resuspensions? $\endgroup$
    – Kasey
    Commented Mar 8, 2016 at 15:43
  • $\begingroup$ I see no reason it will cause any issues. $\endgroup$
    – March Ho
    Commented Mar 8, 2016 at 15:44

1 Answer 1

1
$\begingroup$

If it were me...

  • You can resuspend the two separate pellets (A and B) in 10ul each.
  • Collate in one tube (add A to B).
  • Use the remaining 10ul from your desired volume to 'wash out' tube B and add the wash to tube A.

So long as the DNA is from the same sample this is a perfectly reasonable step that should not result in a substantial loss.

$\endgroup$

You must log in to answer this question.

Not the answer you're looking for? Browse other questions tagged .