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I had ethanol precipitated a large amount of DNA (2ml) and had to split the sample in half to spin down because only the microcentrifuge has the correct rotor to spin that fast. I want to get as concentrated of a DNA sample as possible. Is it possible (correct) to combine the resuspended DNA? I wanted a total of 30 ul of extracted DNA. Could I resuspend each of the two sample in 15 ul and then combine them?

What is the best way to go about this. I realize just keeping them separate might be the best option. I also realized I could've lost DNA when transferring the sample to a smaller tube. I did this because I don't believe it is correct to spin for longer at a lower speed...

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  • $\begingroup$ Assuming you are using standard 1.5ml microcentrifuge tubes, it is completely possible to resuspend a dried DNA pellet from an ethanol/isopropanol precipitation. I have resuspended RNA pellets in 10µl water frequently with no issues. $\endgroup$ – March Ho Mar 8 '16 at 15:05
  • $\begingroup$ But then can I combine the resuspensions? $\endgroup$ – Kasey Mar 8 '16 at 15:43
  • $\begingroup$ I see no reason it will cause any issues. $\endgroup$ – March Ho Mar 8 '16 at 15:44
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If it were me...

  • You can resuspend the two separate pellets (A and B) in 10ul each.
  • Collate in one tube (add A to B).
  • Use the remaining 10ul from your desired volume to 'wash out' tube B and add the wash to tube A.

So long as the DNA is from the same sample this is a perfectly reasonable step that should not result in a substantial loss.

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