What are the relative pros & cons of the following two approaches when one wants to insert a known, sequenced plant gene into a target host organism:

  1. de novo gene synthesis vs
  2. plant matter extracted RNA converted to a cDNA library

Specifically my gene of interest is approx. 2000 base pairs so de novo gene synthesis cost works to about $350. (please correct my estimate if I'm wrong)

While this isn't cheap, collecting plant samples, cryopreservation, digestion, extraction etc. aren't easy either.

Any other considerations when / why one may favor one approach over the other?

  • $\begingroup$ Isn't "collecting plant samples, cryopreservation, digestion, extraction etc." also expensive? Think about cost and time. How long would it take to isolate the DNA vs synthesize it? $\endgroup$ – Gergana Vandova Apr 28 '16 at 19:03

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