I was reading a poster about expression boosting for Artemesinin production in yeast and it had this line:
PGAL1 and PGAL10 have been used for the overexpression of all genes from the mevalonate pathway.
What exactly does this mean? I suppose these are promotors? http://www.yeastgenome.org/locus/S000000224/overview
But how exactly does one use them during cloning to boost expression?
Say that you wanted to over-express ERG10, the gene coding for the first enzyme in the MVA pathway. How does one go about using PGAL10 to do this during the cloning steps?
Link to the poster: https://ddd.uab.cat/pub/tfg/2014/119349/TFG_narcisferrerledo.pdf