What is the typical effect of enzyme immobilization on the kinetic parameters of an enzyme's activity?
Can one assume that they'd stay approximately the same or is there a gross change? Any way to estimate the effect?
The native parameters are as follows:
kcat 0.5 1/min Km 0.6 microM [E0] 5 micro M [S] 60 micro M
Could I expect to retain them after immobilization? Or are these too high for an immobilized enzyme?
If it matters, it is a 500 residue enzyme with a MW of approx. 65,000 Da