I am performing research in which I need to extract bone collagen from the foot bones of American Beaver (Castor canadensis). I have read through the literature fairly thoroughly on both the archaeological/anthropological side and the wild life side and have been finding a many tweaks to a fairly simple extraction process, but I still have a few questions.
When processing bone I split the bone and removed the center, which was red/ light pink, (I thought it was the marrow and wanted to make sure that I was getting collagen from only 1 part of the bone to not confuse my isotope data with varying tissues)
There is also some variability across papers in the time that people allow bone to demineralize in a strong acid ranging from 20 mins to upwards of 10 hours. Does anyone have any experience with this and size to which you ground your samples.
The literature has a variety of temps ranging from 58 C to 95C, it proposes to gellatinize the collagen in a weak acid over a variable number of hours (approx pH of 3), does anyone who has experience offer a temp and time that worked for them.
Any help is much appreciated, Thanks