I have recognized that both the forward and reverse transcripts from a genomic location code for protein products. Both do occur/express in the tissue of interest. In order to eliminate by chance probability, I have screened my entire list of genes and there are 6 different examples. I would appreciate if anyone having the knowledge about such exceptions could guide me further.

Is this phenomena of protein being coded from both directions (of a given genomic location) possible? Are there any known examples?

  • 1
    $\begingroup$ Maybe this can help you? It is about what regulates the directionality of a gene and they seem to include 9% of the genes are translated in both directions. Although it is hard to say without knowing about the organism you are talking about (seems to be eukaryotic, since you mentioned splicing). sciencedirect.com/science/article/pii/S1097276514010077 $\endgroup$
    – skymningen
    Apr 11, 2016 at 13:46
  • $\begingroup$ Many thanks, I have read this paper and its really helpful $\endgroup$
    – Trupti
    Apr 12, 2016 at 6:31

1 Answer 1


While overlapping antisense RNAs are quite well known, there are very few examples in which both the RNAs from the pair can code for proteins. However, it is not impossible. I can cite one validated example: RU2S (DCDC2) and RU2AS (KAAG1) pair.

While RU2S is constitutively and ubiquitously expressed, RU2AS is specifically expressed in kidney, bladder, liver, and testis. It is also expressed in certain tumours (Peltz and Dougherty, 1999).

enter image description here

Peltz SW, Dougherty JP. Antisense Translates into Sense. The Journal of Experimental Medicine. 1999;190(12):1729-1732.


You must log in to answer this question.

Not the answer you're looking for? Browse other questions tagged .