1
$\begingroup$

I have had a few biochemistry courses, but I still feel confused and a bit scared each time they try to explain and apply enzyme kinetics or even chemometrics in different situation during class. On our last lecture we had kinetics regarding:

  • Pingpong reactions (with two substrates A, B):

    $V_0 = \dfrac{K_{cat}[E]* [A][B]}{K_m^B [A] * [B] * [A][B]} $

  • General reactions (with two substrates A, B):

    $V_0 = \dfrac{K_{cat}[E]* [A][B]}{K_m^B [A] * K_s^A * [B] * [A][B]} $

And we also had something about analyzing the "principle component" in multivariate data, from enzyme kinetic measurements (as effect of e.g. pH of temperature).

I really don't understand this, or how I would apply this - or analyze my data if I have two substrates.

Question: In general, is there a good web site which goes beyond the normal Michaelis menten analysis, or a very good book which explains both the basic and more complex scenarios regarding analysis/application of enzyme kinetics for biochemists?

Improve this question
$\endgroup$
2
  • 1
    $\begingroup$ Is there a textbook for your course? If so, which one? At the undergraduate level, Voet & Voet is the most comprehensive for my money (and that is a lot of money). However, for detailed discussion of kinetic mechanisms, like Dan Koshland's ping-pong model, you probably need a book focusing exclusively on enzyme kinetics (not a general textbook) $\endgroup$ – mdperry Apr 21 '16 at 11:51
  • 1
    $\begingroup$ there is no textbook, just the teacher drawing on the board. Its okay to memorize exactly what they say, and then just paste it in on an exam.. but i would really like to understand this. Thank you for your suggestions! $\endgroup$ – CuriousTree Apr 21 '16 at 15:21
3
$\begingroup$

I always used the book "Enzyme Structure and Mechanism" by Alan Fersht. (Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding is the latest version, published in 1998.) Both in some courses I did and when working on (complex) enzymatic mechanisms. It's a classic (so a bit old maybe), but it covers all the basics and includes most of the common complex mechanisms like ping-pong. I don't know if it will teach you how to analyze multivariate data, so maybe you need something else for that.

Improve this answer
$\endgroup$
1
  • 1
    $\begingroup$ Yes, thank you for mentioning this one. It is excellent, and it is a classic. I have an older version somewhere but I could not remember the name. $\endgroup$ – mdperry Apr 21 '16 at 17:59

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.