Do people use random mutagenesis (say using UV) to generate host variants that have high expression of a metabolite / enzyme? I've seen it mentioned as a strategy but it confuses me as to why.
How does that compare to using Directed Evolution for the same purpose? Intuitively, random mutagenesis seems highly inefficient (since you have no control over which genes you mutate) given the combinatorial explosion even in something like E Coli's genome size. Am I making a mistake in this reasoning?
What are situations where one may prefer random mutagenesis over directed evolution? Are there any pros and cons to consider?