What is the highest number of genes expressed under one T7 promoter? Also, what is the longest polycistronic mRNA? I am interested in the context of heterologously expressing biosynthetic gene clusters in E. coli.

  • $\begingroup$ does T2A/P2A peptides work for your case? $\endgroup$ – aaaaaa Apr 25 '16 at 22:28
  • $\begingroup$ @aaaaaa Not really, since I would like to avoid adding extra sequence to the coding region. $\endgroup$ – Gergana Vandova Apr 26 '16 at 20:06

Your Answer

By clicking "Post Your Answer", you acknowledge that you have read our updated terms of service, privacy policy and cookie policy, and that your continued use of the website is subject to these policies.

Browse other questions tagged or ask your own question.