What is the highest number of genes expressed under one T7 promoter? Also, what is the longest polycistronic mRNA? I am interested in the context of heterologously expressing biosynthetic gene clusters in E. coli.

  • $\begingroup$ does T2A/P2A peptides work for your case? $\endgroup$ – aaaaa says reinstate Monica Apr 25 '16 at 22:28
  • $\begingroup$ @aaaaaa Not really, since I would like to avoid adding extra sequence to the coding region. $\endgroup$ – Gergana Vandova Apr 26 '16 at 20:06

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