I want to do respiration process of an animal cell media in laboratory environment.

I don't entirely sure how to supply it with Oxygen to the process because it is a gas, but not a liquid.

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    $\begingroup$ You want to grow animal cells in cell culture flasks? $\endgroup$ – Chris May 3 '16 at 10:28
  • $\begingroup$ @Chris I wanted to grow them in petri dishes, but I'll check it out! $\endgroup$ – Cuba May 3 '16 at 10:32
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    $\begingroup$ That's basically the same. $\endgroup$ – Chris May 3 '16 at 10:36
  • $\begingroup$ @Chris so yes, I consider it as laboratory environment. I just look for a technique of supply. $\endgroup$ – Cuba May 3 '16 at 10:38
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    $\begingroup$ @CubaQ it sounds as if you have little or no experience with growing animal cells in culture. Be aware that there is a lot to know, and if you do things wrong (even slightly wrong) the cells will die very rapidly. This isn't something you can improvise off the top of your head. $\endgroup$ – iayork May 3 '16 at 12:53

Supply of cells growing in adherent culture is unproblematic, as long as you don't fill too much media in the petri dish/cell culture flask.

Oxygen supply here work by diffusion which is getting more problematic if the layer of media covering the cells gets to thick. I usually follow the recommendations of Corning for the amount of media to be used in the flasks/dishes:

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The complete information can be found here, other companies have similar data sheets available. With these amounts I do a lot of cell culture work and I have no growth problems.

  • $\begingroup$ I used to put 4ml of media in t-25 and 9ml in t-75, and change it in ~2 days. I mostly handled HeLa, HEK, Neuro2a and U87 cells. Worked fine for me. $\endgroup$ – WYSIWYG May 3 '16 at 14:38

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