I am new to the CRISPR Cas9 genome editing system and I have the above basic question.
Another way to think about this: what would happen if after cells are transfected, the cultures are left to grow without any selection. Thus, if a mutation disrupts a cell cycle gene (e.g. p53), such cells will proliferate more and the culutre will be populated by those cells rather than healthy cells.
Is this a valid reasoning?