As part of an experiment that I am preparing, it would seem necessary for me to tag a restriction enzyme (HaeIII to be exact) with GFP. I began researching its domains to determine whether to tag the GFP to the N- or C-Terminus, but I was unable to find any previous studies where this restriction enzyme was tagged. As I got asking around, it would seem that no one has ever tagged any restriction enzyme at all.
It appears that this is only the case with restriction enzymes, because I very quickly found several articles where the HaeIII methyltransferase was tagged with GFP.
So, does anyone here know if a restriction enzyme has ever been tagged before? Is there something that I am overlooking that would inhibit the tagging of a restriction enzyme?
Thank you.