I am designing a biosensor, and I need to know whether prokaryotic transcription involves or can involve (if a gene needs to be regulated) enhancer regions. Also, where are enhancer regions located (how far away from the promotor), and when isolating a promotor region, how do you make sure you are also isolating these enhancer regions?

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    $\begingroup$ This question is too broad. Prokaryotic also have enhancers and repressors, of different kinds, their distance from the promoter may vary a lot. If you can list a particular gene you are interested in then I can have a look. In general, however, the right choice of promoter is often enough for a biosensor. $\endgroup$ – alec_djinn Jun 9 '16 at 14:25
  • $\begingroup$ I am deriving the promotor from a silver resistance mechanism found on plasmid pMG101. The SilE is transcriptionally controlled by enhancers known as silS and silR (they are sensor / enhancer responder units). What I need is this promotor with neighbouring enhancer regions. I am not sure how to do this.... $\endgroup$ – Adam Radek Martinez Jun 10 '16 at 12:27
  • $\begingroup$ Good, you should edit your question then. $\endgroup$ – alec_djinn Jun 10 '16 at 12:33
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    $\begingroup$ You can find the sequence of the plasmid, with all the detailed coding regions, here: ncbi.nlm.nih.gov/nuccore/AF067954 . Once you have the sequence you should design a pair of PCR primers to take out what you need. $\endgroup$ – alec_djinn Jun 10 '16 at 12:34
  • $\begingroup$ Dang it, primer design. Thanks for your help! $\endgroup$ – Adam Radek Martinez Jun 10 '16 at 16:53

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