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For the planning of an experiment I would like to know the binding kinetics (kon and koff) of a 6x-His-tag to a Ni-NTA surface.

I know they should differ due to surface quality and position of the His-tag in the protein. But maybe there is some literature out there (that someone of you knows) from which one could get at least a rough estimate of the kinetics.

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  • $\begingroup$ What sort of research have you done so far? Both Entrez PubMed and Google Scholar have very good search functions. $\endgroup$ – MattDMo Jun 14 '16 at 22:33
  • $\begingroup$ It seems I didn't look thoroughly enough at the results PubMed and Scholar returned yesterday. Hope it helps someone in the future… $\endgroup$ – aretaon Jun 15 '16 at 7:22
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Some more hours of literature research finally produced a result:

As published by Khan et al. 2006 6x-His-tag has two distinct dissociation constants of koff1 = 6.3 10-3 and koff2 = 1.8 10-3.

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