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I am looking for a system with which I can deliver (large quantities of) ssDNA in vivo (say in mice). I do not know whether it is of importance but the 5' end of the ssDNA fragments are assumed to be phosphorylated.

If you could suggest some efficient delivery systems to me I would be very delighted.

Many thanks for your help in advance!

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  • $\begingroup$ Small ssRNA can be delivered in-vivo using a peptide from rabies virus (1, 2; it is quite specific to the brain, though). It should work with ssDNA as well but I don't know of an experiment that has tried it. $\endgroup$
    – WYSIWYG
    Jun 15, 2016 at 19:32
  • $\begingroup$ Electroporation! $\endgroup$
    – Luigi
    Jun 16, 2016 at 0:48
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    $\begingroup$ @user19099 In vivo electroporation is possible but it is very localized. $\endgroup$
    – WYSIWYG
    Jun 16, 2016 at 8:13
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    $\begingroup$ @user19099 In vivo electroporation is simple for intramuscular application, just inject the DNA, and then zap the muscle with the electrode. I've seen a paper that used an endoscope to do electroporation inside pig intestine with decent results. Not so good with intravenous injection though. What OP is asking is basically an entire research program. $\endgroup$
    – user137
    Jun 16, 2016 at 9:43
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    $\begingroup$ I would also look at hydrodynamic injection, which is good for delivery to hepatocytes, but not very practical for any other organ. $\endgroup$
    – user137
    Jun 16, 2016 at 9:51

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