Assume we are given approx. 80,000 dsDNA fragments of 40 bps length each. I would like to amplify of each of the 80,000 fragments just one particular strand, such that I obtain amplified 80,000 ssDNA fragments of just 40 bps.
Also beforehand if could be helpful to amplify the 80,000 dsDNA fragments, so where the amplified fragments stay double-stranded.
If you could help me for both amplification steps, particularly the first one, I would be very delighted!
Many thanks in advance!