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I primarily work on animal tissue but I thought of running an SDS-PAGE for plant tissue just for fun. I searched for some protein extraction protocols online and I don't know which one to choose. One is the acetone trichloroacetic acid method where you use the precipitate (?) and chlorophyll stays in the supertanant while the other is a QB (full form?) extraction buffer which is similar to what we use in animal tissue (with a bit of DTT and PVP). Here, we use the supertanant after centrifugal ion and the chlorophyll stays in the loading sample(?). At least, this is what I gather. What are the functional differences and when to use which technique?

Since I'm planning to run a gel, which one would be the better technique?

Please correct me if wrong I'm new to this field

Protocols attached.

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  • $\begingroup$ I can't find the mention of QB buffer in any of the linked protocols. BTW, acetone is used to remove lipids. $\endgroup$ – WYSIWYG Jun 27 '16 at 11:18
  • $\begingroup$ The extraction buffer itself is called the QB buffer I guess @WYSIWYG $\endgroup$ – Polisetty Jun 27 '16 at 21:43

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