I primarily work on animal tissue but I thought of running an SDS-PAGE for plant tissue just for fun. I searched for some protein extraction protocols online and I don't know which one to choose. One is the acetone trichloroacetic acid method where you use the precipitate (?) and chlorophyll stays in the supertanant while the other is a QB (full form?) extraction buffer which is similar to what we use in animal tissue (with a bit of DTT and PVP). Here, we use the supertanant after centrifugal ion and the chlorophyll stays in the loading sample(?). At least, this is what I gather. What are the functional differences and when to use which technique?
Since I'm planning to run a gel, which one would be the better technique?
Please correct me if wrong I'm new to this field