I am reading a patent where they isolate a gene from cDNA constructed from RNA extracted from plant matter.
The codon optimization I can understand but this part confuses me "...remove the first 23 codons from the DNA sequence and replace [the start?] by the ATGGCT sequence."
Why would one remove a set of codons at the start of a sequence? How did they decide how many to remove Is there an obvious reason I am missing? Also what is special about the ATGGCT sequence.
Any ideas why they would do this?
The start of the new sequence (1650 bp) looks like this:
atggctaccg ataatgacag ctctgaaaac cgtcgtatgg gtaattacaa gccgtccatc 60 tggaactacg acttcctgca gtccctggct acccgccaca atatcatgga agagcgccac 120
whereas the original sequence (1710 bp) was this:
atggattctt ccaccgccac cgccatgaga gctccattca ttgatcatac tgatcatgtg 60 aatctcagaa ctgataacga ttcctcagag aatcgaagga tggggaatta taaacccagt 120
Another point I am confused about is that 1710 - 23 x 3 + 6 is 1647. But the new seq. is 1650 bp. What gives?