What is the good or stringent parameter for variant calling?
At present using the DP > 10 and Q > 30 for Variant calling. Is it ok?
We will be needing some additional information to answer your question more completely:
What sequencing platform are you using?
What type of sequencing (as noted by @CMosychuk):
What type of variant caller (as noted by @CMosychuk):
QUAL metric is heavily dictated my sequencing depth, so if you use
30 (phred-scaled), that would be an accuracy of
QUAL metric used by most sequencing studies is
20 so using
30 may cause accumulation of false negatives. But again, depends on the type of sequencing and platform used.