I've been searching for a list of the types of DNA sequencing (e.g. Sanger, Next-Generation) and how prone they are to sequencing errors (None, Somewhat, Very), but I haven't been able to find anything. Here are a few scenarios:
- I have a bacterial culture I want to sequence to see if a plasmid was properly transformed.
- I want to verify a PCR product.
- I want to sequence a large organism's genome.
- I want to verify a mutation found in a human tumor (found using exome data)
Given these examples, and any other you use regularly in the lab, can you please give me information on overall platforms (not specific like Illumina, but general like Sanger sequencing) and their error prone-ness?