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I am going through a paper where they have done DNA-cellulose chromatography to study the interaction between protein and DNA. They are adding the protein to the column and eluting with high salt concentration.

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DNA-cellulose chromatography is used to purify proteins that bind DNA (Potuzak and Dean 1978, Abdullah et al., 1985).

The idea behind it is quite simple: you attach DNA to a cellulose matrix and keep conditions so that the DNA does not dissociate from the cellulose. This stationary phase is then used as a bait for DNA-binding proteins from the mobile phase. The purified DNA-binding proteins can subsequently be dissociated from the column with high salt concentrations.

The method was (as far as I know) first described by Litnam (1968) who used it to extract a DNA polymerase from Micrococcus luteus.

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